Effects of Quercetin on Brain Architecture in Lipopolysaccharide-Induced Neuroinflammation in Mice
DOI:
https://doi.org/10.26538/tjdr/v2i6.1Keywords:
Brain, Quercetin, Feulgen stain, Silver stain, NeuroinflammationAbstract
Purpose: Neuroinflammation is associated with oxidative stress, thereby leading to neuronal dysfunctions in the central nervous system. Quercetin is a well-known potent anti-oxidant capable of reducing the generation of free radicals. Preventing the generation of free radical may be a valuable therapeutic approach in the management of disorders such as Parkinson’s disease, multiples sclerosis and Alzheimer’s disease associated with neuroinflammation. This work was therefore designed to investigate the effect of quercetin on brain architecture in lipopolysaccharide (LPS) induced neuroinflammation in mice.
Method: Fifteen (15) animals (mice) were randomly divided into a group of three consisting of five animals in each group. Neuroinflammation was induced by a single injection of 2 mg/kg Escherichia coli bacterial lipopolysaccharide (serotype 055:B5, Sigma, St Louis, MO, USA) dissolved in 0.9% sterile saline intra-peritonially (IP).
Group A served as the control group and was administered distilled water for a period of three days while group B received only LPS meanwhile group C was induced and treated with quercetin (40 mg/kg p.o) for three days. After three days, all animals were sacrificed, and their brain tissues were carefully collected for analysis. Dendritic arborization and deoxyribonucleic acid (DNA) were quantified using silver stain and feulgen stain respectively
Results: Silver stain revealed that quercetin in group C prevented axonal loss as compared to untreated group B. Meanwhile in the feulgen stain, the DNA was preserved by quercetin as compared to untreated group B.
Conclusion: This study has clearly revealed that quercetin prevented neuronal dysfunction during neuroinflammation through mechanisms that may involve preventing axonal and DNA loss.
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